Vaginal fluid urea and creatinine for diagnosis of premature rupture of membranes

Abstract

To detect the accuracy of vaginal fl uid urea and crea nine for diagnosis of premature rupture of membranes (PROM). Pa ents and methods. The current diagnos c accuracy test was conducted at Ain Shams University Maternity Hospital during the period between June 2011 to December 2011. One hundred women (100) were included in this study and studied women were divided into two groups; group I (cases): fi y pregnant women with PROM were included and group II (controls): fi y pregnant women without PROM were included. Women with mul ple pregnancies, preterm labour, fetal distress, vaginal bleeding, congenital fetal malforma ons, and/or serum crea nine level more than 0.9 mg/dl were excluded from this study. All women were subjected to trans-abdominal ultrasound and sterile Cusco speculum examina on to diagnose PROM and 5ml of sterile saline solu on was injected into the posterior vaginal fornix using a sterile syringe. Three ml of the injected saline was aspirated using the same syringe and sent immediately to the laboratory. Each specimen was centrifuged at 50 revolu ons/ second and the supernatant fl uid was separated. Measurements of both urea and crea nine were performed by enzyma c urease method and Rate Jaff e method respec vely to determine their exact levels.