Abstract:
The rapid growth of the global biopharmaceutical market in recent years indicates its
importance in the biotechnology industry. The production of these medicinal products
is one of the fastest-growing industries in the patient treatment field. Since 1980,
processes have been developed to optimize the production of recombinant protein
products upstream and downstream. In this regard, one promising approach is the
engineering of expression vectors based on combinations of DNA regulatory elements
found in euchromatin regions. This approach aims to achieve proper gene integration
and facilitate its expression in the target cell, as epigenetic mechanisms can lead
to instability of the desired gene in long-term cell cultures and gene silencing. To
address this issue, genetically engineered vectors have been produced, which include
components such as Matrix Attachment Regions (MARs), Scaffold Attachment Regions
(SARs), Stabilizing Anti-Repressor Elements (STAREs), and Ubiquitous Chromatin
Opening Elements (UCOE). These components can modify the chromatin environment
to minimize gene silencing and enhance higher, more stable, and biologically active
expression of recombinant molecules. In this review, we focus on different approaches
and developments in the technology of expression vector engineering and their impact
on increasing the production of recombinant proteins
Description:
The rapid growth of the global biopharmaceutical market in recent years indicates its
importance in the biotechnology industry. The production of these medicinal products
is one of the fastest-growing industries in the patient treatment field. Since 1980,
processes have been developed to optimize the production of recombinant protein
products upstream and downstream. In this regard, one promising approach is the
engineering of expression vectors based on combinations of DNA regulatory elements
found in euchromatin regions. This approach aims to achieve proper gene integration
and facilitate its expression in the target cell, as epigenetic mechanisms can lead
to instability of the desired gene in long-term cell cultures and gene silencing. To
address this issue, genetically engineered vectors have been produced, which include
components such as Matrix Attachment Regions (MARs), Scaffold Attachment Regions
(SARs), Stabilizing Anti-Repressor Elements (STAREs), and Ubiquitous Chromatin
Opening Elements (UCOE). These components can modify the chromatin environment
to minimize gene silencing and enhance higher, more stable, and biologically active
expression of recombinant molecules. In this review, we focus on different approaches
and developments in the technology of expression vector engineering and their impact
on increasing the production of recombinant proteins
