Аннотации:
he goal of this study is to improve methodologies that define the maturation of ovarian follicles and enhance in vitro fertilization by employing decellularized ovaries. Preantral follicles of mice were cultured for 14 days in both the decellularized ovary and two- dimensional (2D) conditions. The oocyte maturation rate, fertilization rate, and the subsequent embryo development rate were assessed in 2D and the decellularized ovary and compared to in vivo condition. Additionally, the gene expression profile of IGF1R, integrin αvβ3, Cox2, Caspase-3, Bax, and Bcl2 l1 was determined in blastocysts. The culture in the decellularized ovary showed a significantly higher number of MII oocytes in comparison to the 2D culture (P < 0.05). Compared to in vivo, both the 2D and the decellularized ovary cultures exhibited significantly lower percentages of MII oocytes, 2PN, two-cell, cleavage, and blastocyst (P < 0.05). In the decellularized ovary culture, significantly higher percentages of 2PN and blastocyst were observed (P < 0.05) compared to the 2D culture. The gene expression level of IGF1R and Cox2 in blastocysts from both the 2D and the decellularized ovary cultures was markedly lower compared to in vivo. However, the gene expression levels of Integrin αv and β3 were comparable in blastocysts derived from in vivo and decellularized ovary-matured oocytes. Blastocysts derived from decellularized ovary-matured oocytes showed a higher bcl211 expression level compared to the blastocysts from 2D (P < 0.05). Employing decellularized ovarian tissues methodologies for in vitro maturation of oocytes provides a promising avenue towards generating embryos with improved implantation potential.
Описание:
he goal of this study is to improve methodologies that define the maturation of ovarian follicles and enhance in vitro fertilization by employing decellularized ovaries. Preantral follicles of mice were cultured for 14 days in both the decellularized ovary and two- dimensional (2D) conditions. The oocyte maturation rate, fertilization rate, and the subsequent embryo development rate were assessed in 2D and the decellularized ovary and compared to in vivo condition. Additionally, the gene expression profile of IGF1R, integrin αvβ3, Cox2, Caspase-3, Bax, and Bcl2 l1 was determined in blastocysts. The culture in the decellularized ovary showed a significantly higher number of MII oocytes in comparison to the 2D culture (P < 0.05). Compared to in vivo, both the 2D and the decellularized ovary cultures exhibited significantly lower percentages of MII oocytes, 2PN, two-cell, cleavage, and blastocyst (P < 0.05). In the decellularized ovary culture, significantly higher percentages of 2PN and blastocyst were observed (P < 0.05) compared to the 2D culture. The gene expression level of IGF1R and Cox2 in blastocysts from both the 2D and the decellularized ovary cultures was markedly lower compared to in vivo. However, the gene expression levels of Integrin αv and β3 were comparable in blastocysts derived from in vivo and decellularized ovary-matured oocytes. Blastocysts derived from decellularized ovary-matured oocytes showed a higher bcl211 expression level compared to the blastocysts from 2D (P < 0.05). Employing decellularized ovarian tissues methodologies for in vitro maturation of oocytes provides a promising avenue towards generating embryos with improved implantation potential.
